Abstract:
In this study, a multi-functional study on the endophytic strains of strawberry was carried out using plate confrontation method, UV spectrophotometric method and 24-hole culture method. A multi-functional strawberry endophytic strain SRF5 with antagonistic function, autotoxins degradation function and nematicidal function was obtained to prevent and control strawberry replant disease. The results showed that the inhibition rate of the screened strain SRF5 on Fusarium oxysporum was 71.65%. At day 7, the degradation rates of SRF5 for para-hydroxybenzoic acid and benzoic acid were above 90% and 60%, respectively.The corrected mortality rate of SRF5 for Meloidogyne incognita reached 97.37% after 48 h of treatment，and the egg hatching rate reduced by 61.33% at day 7 comparing with the control group. According to the results of morphology and molecular biology analysis, the ITS sequence of SRF5 demonstrated 100% homology with the Neosartorya udagawae(JQ316519.1) sequence. Therefore, the screened strain was preliminarily identified as Neosartorya with potential development and application value.
