Chronic bacterial prostatitis (CBP) is a common male urinary disease,while bone marrow mesenchymal stem cells (BMSCs) have the characteristics of accumulation in inflammatory tissues and secreting anti-inflammatory substances.On these basis, ultrasound combined with microbubbles (USM) was used to open the blood-prostate barrier (BPB) to enhance the CBP treatment effect of BMSCs carrying curcumin micelles (BMSCs-CM). BMSCs isolation and identification were performed by density gradient centrifugation and surface antigen detection. The cell survival and intracellular curcumin fluorescence quantification were carried out to select the optimal parameters for constructing BMSCs-CM. Fluorescence imaging was used to show the targeted enrichment of USM assisted BMSCs-CM in prostate tissue. The efficacy of USM assisted BMSCs-CM on CBP was evaluated by prostate index and inflammatory factor levels. Results showed that the combined incubation of 100 μg/mL CM and BMSCs for 4 h was the optimal condition for constructing BMSCs-CM;USM could significantly promote the enrichment of BMSCs-CM in prostate tissue, and USM assisted BMSCs-CM treatment could effectively reduce the inflammation of CBP. Our finding provides a new idea for the clinical treatment of CBP.

ultrasound microbubble; blood-prostate barrier; bone marrow mesenchymal stem cells; curcumin; chronic bacterial prostatitis