Abstract:
Coenzyme Q10 (CoQ10) is one of the most important evaluation index for the metabolism ability of acetic acid in acetic acid bacteria. In this investigation, a reversed-phase high performance liquid chromatography (RP-HPLC) method was established to determine the content of CoQ10 in acetic acid bacteria. It was performed on a Diamonsil C18 column (4.6 mm×250 mm, 5 μm) with column temperature setting at 35 ℃. Mobile phase was composed with methanol and ethanol in a volume ratio of 20∶80. The flow rate was 1.0 mL/min and the detective wavelength was 275 nm. Under these conditions, the calibration curve of CoQ10 demonstrated good linear relationship in the range of 1~100 μg/mL (R2=0.999 2) with the relative standard deviation (RSD) of 2.93% and the average recovery rate of 97.07% , proving the effectiveness of this method in determining CoQ10 content in acetic acid bacteria. On the basis of single factor experiments, a quadratic polynomial rgression equation of CoQ10 extraction in acetic acid bacteria was constructed using the response surface methodology. The optimal extraction conditions were established as follow: the temperature was 91 ℃, the concentration of hydrochloric acid was 0.08 mol/L, the extraction time was 38 min. In these optimized conditions, the content of CoQ10 extracted from acetic acid bacteria (114.81 μg/g) was close to the theoretical value (115.66 μg/g), and was 368.83% higher than the extracted value before optimization. The results can be used to further utilize acetic acid bacteria and develop functional CoQ10 products.