外源性VD3缓解运动诱导的慢性疲劳综合症的机制-陕西师范大学学报期刊社网站

陕西师范大学学报（自然科学版）

抗癌机制与人类健康专题

外源性VD3缓解运动诱导的慢性疲劳综合症的机制

吴方南1，呼德尔朝鲁1,2，田振军1*

(1 陕西师范大学体育学院，陕西西安 710119；2 内蒙古体育职业学院，内蒙古呼和浩特 010051)

田振军，男，教授，博士生导师,研究方向为慢性疾病与运动干预。E-mail：tianzj611@hotmail.com

摘要:

研究了补充VD3缓解运动诱导的慢性疲劳综合症(CFS)的机制。将80只小鼠随机分5组(n=16)，即安静对照组(C组)、CFS造模组(CFS组)、VD3组又分为造模前组(bVD组)、过程中组(mVD组)和造模后VD干预组(aVD组)。造模、干预持续4周，全程行为学观察，4周后进行力竭测试和悬尾实验，再迅速采血并切取股四头肌组织。测定血睾酮、血清皮质酮及股四头肌组织的MDA、T-AOC、GSH-PX和T-SOD，采用Western Blotting测定SIRT1、SIRT3、ERK1/2、p-ERK1/2和MEK1/2。结果表明：与C组比较，CFS组小鼠打斗损伤严重，血睾酮及MEK、SIRT3显著降低(P<0.05)，皮质酮、MDA、T-SOD、GSH-PX显著增加(P<0.05)；与CFS组比较，VD干预组小鼠打斗损伤较轻，皮质酮、MDA显著降低(P<0.05)，血睾酮、T-AOC、T-SOD、GSH-PX及MEK、SIRT1、SIRT3和p-ERK/ERK均显著增加(P<0.05)。外源性补充VD3可提高骨骼肌抗氧化能力，缓解运动诱发的CFS症状，其机制可能是通过激活MEKs/ERKs-SIRT1信号通路，降低MEK表达，增加ERK磷酸化水平，上调SIRT1和SIRT3表达而实现。

关键词：

VD3；游泳运动；慢性疲劳综合症；骨骼肌；氧化应激

收稿日期：

2018-11-17

中图分类号：

G804

文献标识码：

文章编号：

1672-4291(2020)01-0106-08

基金项目：

国家自然科学基金（31040045）

Doi:

Mechanism of exogenous VD3 supplement alleviates the exercise-induced chronic fatigue syndrome

WU Fangnan1, HUDER Chaolu1,2, TIAN Zhenjun1*

(1 School of Physical Education, Shaanxi Normal University, Xi′an 710119, Shaanxi, China;2 Inner Mongolia Vocantional College of Physical Educaiton, Hohhot 010051, Inner Mongolia, China)

Abstract:

The exogenous VD3 supplement alleviates exercise-induced chronic fatigue syndrome (CFS) and its mechanism are investigated.80 male mice were randomly divided into 5 groups(n=16)，named quiet control group (C), CFS model group (CFS), VD supplement group before(bVD), during(mVD)and after (aVD) modeling.The modeling and supplement continued 4 weeks while behavior observation were achived. After 4 weeks, the tail suspension test and the forced swimming test were implemented, then the blood and quadriceps were dissected rapidly. Serum testosterone and corticosterone content, as well as MDA, T-AOC, GSH-PX and T-SOD in the quadriceps were measured. Western blotting was used to measure SIRT1, SIRT3, ERK1/2, p-ERK1/2 and MEK1/2.Compared with C group, CFS group appeared traces of fighting, and the concentration of serum testosterone, MEK and SIRT3 decreased significantly (P

KeyWords:

VD3; swimming exercise; chronic fatigue syndrome; muscle; oxidative stress