自然科学版
陕西师范大学学报(自然科学版)
抗癌机制与人类健康专题
长链非编码RNA NONHSAT068183在肾透明细胞癌组织中的表达及其临床意义
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冯雪1,陶志伟1,蒋思雄2,郑善亮1,孙卫兵2,刘淑清1*,孙明忠1*
(1 大连医科大学 基础医学院,辽宁 大连 116044; 2 大连医科大学 第二附属医院,辽宁 大连 116044)
孙明忠,男,教授,博士生导师,主要从事肿瘤转移分子机制及临床相关性研究。E-mail:smzlsq@163.com刘淑清,女,教授,博士生导师,主要从事肿瘤转移的机制研究。E-mail:lsqsmz@163.com
摘要:
通过研究长链非编码RNA NONHSAT068183(lnc068183)在肾透明细胞癌(ccRCC)患者组织中表达水平的变化,分析其表达水平与ccRCC临床指标间的相关性。实验收集55例ccRCC肿瘤组织及其对应的癌旁正常组织,分别设为ccRCC组和对照组;采用qRT-PCR方法检测lnc068183在ccRCC组及对照组组织中的表达水平,用t检验分析其水平变化与ccRCC患者临床指标间的相关性及意义。与对照组相比,lnc068183在ccRCC组肿瘤组织中的表达水平显著降低(P<0.000 1)。在临床诊断指标中,ccRCC肿瘤组织中lnc068183的表达水平与ccRCC患者的TNM分期(P=0.005)及Fuhrman分级(P=0.015)呈负相关;ROC分析显示lnc068183对ccRCC有较明确的辅助诊断意义。作为抑癌基因,lnc068183在ccRCC中表达下调,可能与ccRCC的发生和发展相关,为肾癌诊治及研究提供了潜在的新线索。
关键词:
肾透明细胞癌;长链非编码RNA NONHSAT068183;抑癌基因
收稿日期:
2018-10-16
中图分类号:
R737.1
文献标识码:
A
文章编号:
1672-4291(2020)01-0087-05
基金项目:
国家自然科学基金(81672737, 81272186, 81171957);辽宁省自然科学基金(2015020266, 2014023047)
Doi:
The expression and clinical significance of lncRNA NONHSAT068183 in clear cell renal cell carcinoma
FENG Xue1, TAO Zhiwei1, JIANG Sixiong2, ZHENG Shanliang1, SUN Weibing2, LIU Shuqing1*, SUN Mingzhong1*
(1 Basic Medical College, Dalian Medical University, Dalian 116044, Liaoning, China; 2 The Second Affiliated Hospital of Dalian Medical University, Dalian 116044, Liaoning, China)
Abstract:
The expression level of long non-coding RAN NONHSAT068183 (lnc068183) in tumorous tissues from clear cell renal cell carcinoma (ccRCC) was measured in order to investigate the association between lnc068183 level change and ccRCC clinicopathological parameters. Clinical tumorous tissues and paracancerous normal tissues from 55 ccRCC patients were collected and classified as ccRCC group and control group. Lnc068183 level was then determined and quantified by qRT-PCR assay, and t test analysis was performed to reveal the relationship and significance of lnc068183 level change with clinicopathological parameters of ccRCC patients. Compared with the control group, lnc068183 level was significantly decreased (P<0 .000 1) in tissues from ccRCC group. Concerning the clinical diagnostic parameters, lnc068183 expression level in tumorous tissues was negatively correlated with TNM stage (P="0.005)" and Fuhrman grade (P="0.015)" of ccRCC patients. ROC curve showed lnc068183 was in potential diagnostic use in ccRCC diagnosis. As a tumor suppressor gene, the downregulation of lnc068183 in patient tumorous tissue is potentially associated with the occurrence and development of ccRCC, providing a new clue for the diagnosis, treatment and research of renal cancer.
KeyWords:
clear cell renal cell carcinoma; lncRNA NONHSAT068183; tumor suppressor gene