玛咖对运动疲劳大鼠纹状体Glu、GABA含量以及PV阳性神经元和VGLUT1表达的影响-陕西师范大学学报期刊社网站

陕西师范大学学报（自然科学版）

运动生物学专题

玛咖对运动疲劳大鼠纹状体Glu、GABA含量以及PV阳性神经元和VGLUT1表达的影响

王志锋1,2，乔德才1*, 郭琪2，闫清伟3

（1 北京师范大学体育与运动学院, 北京 100875; 2 西安工程大学体育部，陕西西安 710048；3 西藏民族大学体育学院，陕西咸阳 712082）

乔德才,男,教授,博士生导师,主要研究方向为运动生理学和神经生物学。Email:decaiq@bnu.edu.cn

摘要:

采用高效液相色谱技术对灌胃玛咖(Maca)后运动疲劳大鼠纹状体Glu和GABA含量进行检测，并采用免疫荧光技术检测PV阳性神经元和VGLUT1的表达，探讨玛咖(Maca)对运动疲劳大鼠纹状体谷氨酸（Glu）和γ氨基丁酸（GABA）含量以及小清蛋白（PV）阳性神经元和谷氨酸转运体1 （VGLUT1）表达的影响。结果表明：运动疲劳后大鼠纹状体Glu和GABA含量极显著升高（P＜0.01），灌胃Maca后Glu和GABA含量极显著和显著降低（P＜0.01和P＜0.05）。运动疲劳后大鼠纹状体VGLUT1和PV阳性神经元表达均极显著增加（P＜0.01），灌胃Maca后VGLUT1表达显著降低（P＜0.05），PV阳性神经元表达有降低趋势（P＞0.05）。结果提示：Maca可能通过抑制VGLUT1和PV阳性神经元的表达降低运动疲劳大鼠纹状体Glu和GABA的含量，并起到延缓运动疲劳的作用。

关键词：

玛咖；大鼠；纹状体；谷氨酸；γ氨基丁酸；PV阳性神经元；VGLUT1

收稿日期：

2018-03-12

中图分类号：

G804.7

文献标识码：

文章编号：

16724291(2019)03004809

基金项目：

西藏自治区自然科学基金（2016ZR1520）;西安工程大学博士科研启动基金（BS201889）

Doi:

Effects of Maca on Glu, GABA content, PV positive neurons and VGLUT1 expression in striatum of rats with exercise fatigue

WANG Zhifeng1,2, QIAO Decai1*, GUO Qi2, YAN Qingwei3

(1 School of Physical Education and Sports, Beijing Normal University, Beijing 100875, China;2 Department of Physical Education,Xi′an Polytechnic University,Xi′an 710048, Shaanxi,China; 3 College of Physical Education, Xizang Minzu University,Xianyang 712082, Shaanxi,China)

Abstract:

The aim of this paper was to investigate the effects of Maca on the contents of glutamate (Glu) and γ aminobutyric acid (GABA) in striatum of rats with exercise fatigue, and the expression of (PV) positive neurons and glutamate transporter 1 (VGLUT1).The content of Glu and GABA in rat striatum was determined by high performance liquid chromatography (HPLC), and the expression of PV positive neurons and VGLUT1 in rat striatum was detected by immunofluorescence technique after the establishment of exercise fatigue model.The results show that the contents of Glu and GABA in rat striatum increased significantly after exercise fatigue (P＜0.01), and the contents of Glu and GABA decreased significantly after gastric perfusion of Maca (P＜0.01 and P＜0.05).After exercise fatigue, the expression of VGLUT1 and PV positive neurons in rat striatum was significantly increased (P＜0.01).The expression of VGLUT1 was significantly decreased after gastric perfusion of Maca(P

KeyWords:

Maca; rat; striatum; glutamate; gammaaminobutyric acid; PV positive neurons; VGLUT1