Abstract:
To investigate the effects of mitochondrial nutrient α-lipoic acid on mitochondrial antioxidant capacity and energy metabolism marker enzyme activity in rat quadriceps muscle. SD rats were divided into quiet control group (QC group), exercise control group (EC group) and exercise plus α-lipoic acid group (EPL group), with 8 rats in each group. On the last day of the sixth week, the quadriceps muscle was taken and mitochondrial samples were prepared to test the mitochondrial antioxidant index and the marker enzyme activity of mitochondrial function in the rat quadriceps muscle.The results showed that the activities of antioxidant enzymes in EC group and EPL group were lower than those in QC group (P＜0.01 or P＜0.05). Compared with EC group, the activity of antioxidant enzymes in EPL group increased (P＜0.05 or P＜0.01).Compared with QC group, MDA and H2O2 levels in EC group and EPL group were increased (P＜0.01).Compared with EC group, MDA and H2O2 levels in EPL group were decreased (P＜0.01).Compared with QC, the three ATPase activities in the EC group and the EPL group decreased (P＜0.01 or P＜0.05).Compared with the EC group, the three ATPase activities in the EPL group increased (P＜0.05 or P＜0.01).Compared with the QC group, the activity of the functional marker in the EC group decreased (P＜0.05 or P＜0.01), and the activity of the functional marker in the EPL group decreased (P＜0.05). Compared with the EC group, the activity of the functional marker in the EPL group increased (P＜0.05 or P＜0.01). The time from exercise to fatigue in the EPL group was prolonged by 30.05%.The results showed that α-LA had no adverse effects on the weight gain of exercise-trained rats.The structure, function and maintenance of mitochondria ensured the oxidative energy supply efficiency of mitochondria and increased the aerobic exercise level of rats，and has a strong effect of anti-fatigue.
