自然科学版
陕西师范大学学报(自然科学版)
生命科学
三种螳蛉线粒体基因组及脉翅目系统发育分析
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陈涛1,2, 兰旭娥1, 陈晓宁1, 尤平1*
(1 陕西师范大学 生命科学学院,陕西 西安 710119;2 桂林理工大学 资产管理处,广西 桂林 541004)
尤平,男,教授,博士生导师,主要从事鱼类寄生虫学和动物分子生态学方面的研究。E-mail:youping@snnu.edu.cn
摘要:
通过PCR结合引物步移法测定日本螳蛉Mantispa japonica、汉优螳蛉Eumantispa harmandi和铜头螳蛉Euclimacia badia的全线粒体基因组,并进行序列分析。结果表明:日本螳蛉、汉优螳蛉和铜头螳蛉的全线粒体基因组长度分别为16 106、15 741和15 899 bp,基因组成和排列与其他已测脉翅目昆虫一致,均包括13个蛋白质编码基因(PCGs)、22个转运RNA基因(tRNA)、2个核糖体RNA基因(rRNA)和1个长度可变的A+T富集区。蛋白质编码基因除COⅠ以ACG作为起始密码子外,其余基因均以ATN作为起始密码子;除COⅠ、COⅡ、ND5、ND4以不完整的T作为终止密码子外,其他基因终止密码子均为完整的TAA。汉优螳蛉的22个tRNA均形成典型的三叶草形二级结构;而日本螳蛉和铜头螳蛉的21个tRNA形成典型的三叶草形二级结构,仅trnSAGN二级结构缺失DHU臂,在该位置形成1个环。A+T富集区位于rrnS和trnI-trnQ-trnM之间,长度分别为1 281、936和1 092 bp,并在该区域存在一些串联重复序列及1个茎环结构。基于13个蛋白质编码基因数据集,采用最大似然法(ML)和贝叶斯法(BI)对脉翅目进行初步的系统发育分析,结果显示两种系统树拓扑结构一致,符合以往形态学和分子分类的研究结论,支持螳蛉科的单系性,但不支持蚁蛉科的单系性。
关键词:
日本螳蛉;汉优螳蛉;铜头螳蛉;线粒体基因组;脉翅目
收稿日期:
2017-10-11
中图分类号:
Q961
文献标识码:
A
文章编号:
1672-4291(2018)06-0081-12
基金项目:
国家自然科学基金(31872203);陕西省自然科学基金(2017JM3014)
Doi:
Studies on complete mitochondrial genomes and Neuroptera phylogenesis of three Mantispidae
CHEN Tao1,2,LAN Xu′e1,CHEN Xiaoning1, YOU Ping1*
(1 School of Life Sciences,Shaanxi Normal University,Xi′an 710119,Shaanxi,China;2 Department of Asset,Guilin University of Technology,Guilin 541004,Guangxi,China)
Abstract:
Complete mitochondrial genomes (mitogenomes) of Mantispa japonica, Eumantispa harmandi and Euclimacia badia were sequenced and primarily analyzed by PCR with primer walking method. The results show that the mitogenomes of M. japonica, E. harmandi and E. badia are 16 106, 15 741 and 15 899 bp in length, respectively. They contain 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNA), two ribosomal RNA genes (rRNA) and a variable A+T-rich region in same order and orientation as the sequenced mitochondrial genomes of other Neuroptera insects. All PCGs start with the typical ATN codon except for COⅠ which begins with ACG. For stop codon, TAA is used in all the PCGs, while the COⅠ,COⅡ,ND5 and ND4 exist incomplete stop codon T. All tRNAs of E. harmandi have the typical cloverleaf secondary structure, while all tRNAs of M. japonica and E.badia have the typical cloverleaf secondary structure except for trnSAGN which lacks the DHU stem and form a loop. The A+T-rich regions of the three insects in family Mantispidae located between the rrnS and trnI-trnQ-trnM are 1 281, 936 and 1 092 bp in size, in addition, there are some tandem repeats and a stem-loop structure in the A+T-rich regions. Phylogenetic analyses of Neuroptera were assessed based on 13 PCGs dataset using the maximum likelihood(ML) and Bayesian inference (BI) methods, the results show the identical topology structure and accorded with previous morphological and molecular taxonomies of the Neuroptera. The monophyly of the family Mantispidae is well-supported, while the monophyly of the family Myrmeleontidae is not supported.
KeyWords:
Mantispa japonica; Eumantispa harmandi; Euclimacia badia; mitochondrial genomes; Neuroptera