Abstract:
Complete mitochondrial genomes (mitogenomes) of Mantispa japonica, Eumantispa harmandi and Euclimacia badia were sequenced and primarily analyzed by PCR with primer walking method. The results show that the mitogenomes of M. japonica, E. harmandi and E. badia are 16 106, 15 741 and 15 899 bp in length, respectively. They contain 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNA), two ribosomal RNA genes (rRNA) and a variable A+T-rich region in same order and orientation as the sequenced mitochondrial genomes of other Neuroptera insects. All PCGs start with the typical ATN codon except for COⅠ which begins with ACG. For stop codon, TAA is used in all the PCGs, while the COⅠ,COⅡ,ND5 and ND4 exist incomplete stop codon T. All tRNAs of E. harmandi have the typical cloverleaf secondary structure, while all tRNAs of M. japonica and E.badia have the typical cloverleaf secondary structure except for trnSAGN which lacks the DHU stem and form a loop. The A+T-rich regions of the three insects in family Mantispidae located between the rrnS and trnI-trnQ-trnM are 1 281, 936 and 1 092 bp in size, in addition, there are some tandem repeats and a stem-loop structure in the A+T-rich regions. Phylogenetic analyses of Neuroptera were assessed based on 13 PCGs dataset using the maximum likelihood(ML) and Bayesian inference (BI) methods, the results show the identical topology structure and accorded with previous morphological and molecular taxonomies of the Neuroptera. The monophyly of the family Mantispidae is well-supported, while the monophyly of the family Myrmeleontidae is not supported.