Abstract:
Aerobic exercise could influence the concentration of homocysteine (Hcy) in plasma, thereby affecting the incidence of atherosclerosis (AS). In this assay, a chloroacetate caged fluorescein chemodosimeter (CACFC) was designed and synthesized, which employed bis-molecular recognition of mercapto and amine groups to selectively detect Hcy and Cys over other amino acids. The results show that in ethanol water (V/V, 1/10) system, there was a good linearity between the fluorescence intensity of CACFC and the concentrations of Hcy or Cys in the range of 40～280 μmol/L and 20～280 μmol/L, respectively. This sensor could not only achieve fluorescence imaging applications of Hcy in living cells，but also could be used to effectively detect the concentration of Hcy in plasma before and after aerobic exercise in patients with AS. Therefore, the study provide a new research idea for expanding its application in the field of biomedicine.
