To investigate the effect of different sleep deprivations (SD) on the mood,learning and memory of mice, both multiple platform method and sleep deprivation tank method were used to observe the effects of the relative factors on the anxiety-like behavior,learning and memory.The following results were obtained.12 h SD and 24 h SD did not affect anxiety-like behavior, learning and memory in either of the above paradigms. The anxiety-like behavior was observed in mice subjected to 48 h SD via multiple platform method and the spatial memory was not affected，but sleep deprivation tank did not make mice appear anxiety-like behavior and spatial memory ability after 48 h SD. In the sleep deprivation tank experiment, a long-time SD could induce anxiety-like behavior, whereas the learning process of the mice slowed down and spatial memory was impaired after 3 weeks SD. Thus, deprivation tank is advantageous as an SD model for studies on human long-term sleep insufficiency.

sleep deprivation；anxiety-like behavior；learning and memory