黄脸油葫芦精巢TeHsc70和TeHsp90敲低对20E相关基因表达的影响-陕西师范大学学报期刊社网站

陕西师范大学学报（自然科学版）

生命科学

黄脸油葫芦精巢TeHsc70和TeHsp90敲低对20E相关基因表达的影响

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金文杰1, 2, 谭娥1, 王桂荣1, 纪艳青1, 薛竞雄1, 奚耕思1*

(1 陕西师范大学生命科学学院，陕西西安 710119；2 青海大学生态环境工程学院，青海西宁 810016）

奚耕思，女，教授，博士生导师，主要从事昆虫生殖生物学及其系统进化方面的研究工作。E-mail:xigengsi@snnu.edu.cn

摘要:

为了研究Hsc70和Hsp90与ERR和20E下游初、次级应答基因的关联性，明确Hsc70和Hsp90如何参与20E信号通路进而调控雄性生殖，以雄性成体黄脸油葫芦（Teleogryllus emma Ohmachi＆Matsuura）为实验对象，设实验组、阴性对照组和Control组。实验组注射dsTeHsc70和dsTeHsp90；阴性对照组注射EGFP-dsRNA，干扰48、72和96 h后qPCR检测RNA干扰（RNA interference, RNAi）效率和20E相关基因的表达。结果显示，TeEcR、TeERR和TeUsp在空白组成体精巢中高表达，并且TeERR mRNA的表达显著高于TeEcR和TeUsp （P<0.05）。dsTeHsc70+dsTeHsp90干扰96 h后，TeHsc70和TeHsp90 mRNA的表达显著下调（P<0.05），并且TeERR和20E初、次级应答基因TeEcR、TeUsp、TeE75B和TeHR3mRNA的表达也随之极显著下调（P<0.01）。据此推测，TeHsc70、TeHsp90和TeERR均参与20E信号通路，并且TeHsc70、 TeHsp90和TeERR对于20E信号通路维持精巢的发育和形态建成方面必不可少。

关键词：

黄脸油葫芦；精巢；热休克蛋白；RNA干扰；蜕皮激素

收稿日期：

2018-01-22

中图分类号：

Q969.93

文献标识码：

A

文章编号：

1672-4291(2018)03-0087-07

基金项目：

国家自然科学基金(31571276,31171195)

Doi:

Effects of Hsc70 and Hsp90-RNAi on expression of 20E-related genes in testis of Teleogryllus emma

JIN Wenjie1, 2, TAN E1, WANG Guirong1, JI Yanqing1, XUE Jingxiong1, XI Gengsi1*

(1 School of Life Sciences, Shaanxi Normal University, Xi′an 710119, Shaanxi, China; 2 College of Eco-Environmental Engineering, Qinghai University, Xining 810016, Qinghai, China)

Abstract:

To explore the relationship between Hsc70 and Hsp90 with ERR, 20E primary and secondary response genes, and how do the ERR and 20E-related genes involved in the 20E signaling pathway and male fertility, adult male crickets Teleogryllus emma Ohmschi & Matsumura were divided into the experimental group, negative group, and control group. Crickets of the experimental group were injected with dsTeHsc70 and dsTeHsp90, and the negative group received EGFP-dsRNA, then the efficiency of TeHsc70- and TeHsp90-RNAi and expression of TeERR and 20E-related genes were detected in the experimental group after the crickets treated for 48, 72 and 96 h. Results showed that the TeEcR, TeERR, and TeUsp were highly expressed in free-treated adult testes, and TeERR expression was significantly higher than TeEcR and TeUsp (P

KeyWords:

Teleogryllus emma; testis; heat shock proteins; RNA interference; ecdysteroids