FAK调节胃癌、肝癌细胞恶性行为及相关基因表达的体外研究-陕西师范大学学报期刊社网站

陕西师范大学学报（自然科学版）

生命科学

FAK调节胃癌、肝癌细胞恶性行为及相关基因表达的体外研究

程思楠，马妮，肖丽，何慧敏，杨倩，黎金媚，侯依凡，刘政，侯颖春*

(陕西师范大学生命科学学院，陕西西安 710119)

侯颖春，男，教授，博士生导师。E-mail：ychhou@snnu.edu.cn

摘要:

以siRNA技术对人胃癌细胞（SGC7901）和肝癌细胞（SMMC7721）的FAK表达进行抑制以后，通过MTT、损伤修复、Transwell、流式细胞仪、激光共聚焦和电子显微镜等方法对这些细胞的细胞行为的变化进行了研究。同时，以RT-PCR、Western blot、细胞免疫荧光法检测下调FAK表达对Src、Ras、Apex1、Rgnef的表达的影响。结果发现，FAK基因表达被抑制后两种癌细胞运动能力明显降低，细胞增殖力显著下降，Src、Ras、Apex1、Rgnef的mRNA表达水平以及蛋白质的表达水平也明显被抑制。结果表明,在癌细胞的转移、侵袭等过程中FAK有着非常重要的促进作用，而且FAK可以促进Src、Ras、Apex1、Rgnef的表达，这可能有助于基于复杂信号网络理解FAK在肿瘤发生、发展过程中作用。

关键词：

黏着斑激酶；胃癌；肝癌；细胞恶性行为；基因功能

收稿日期：

2017-08-17

中图分类号：

Q279

文献标识码：

文章编号：

1672-4291(2018)02-0074-07

基金项目：

陕西省科技计划项目（SJ08-ZT09）；西安市科技计划项目（YF07188）；陕西省2016春笋计划项目

Doi:

FAK modulating the malignancy and associated gene expressions of

SGC7901 and SMC7721 cells in vitroCHENG Sinan, MA Ni, XIAO Li, HE Huimin, YANG Qian,LI Jinmei, HOU Yifan, LIU Zheng, HOU Yingchun*

（School of Life Sciences, Shaanxi Normal University, Xi′an 710119, Shaanxi, China）

Abstract:

To explore the relationship between FAK expression of cancer cell malignancy, FAK expression was inhibited in SGC7901 and SMC7721 cells.The cell malignancy was checked using MTT, wound healing, Transwell, flow cytometry, laser confocal microscope and electron microscope, and the associated gene expressions were detected using RT-PCR and western blot. The results indicated that down-regulation of FAK decreased the proliferation and motility of SGC7901 and SMMC7721 cells significantly, and the expressions of Src, Ras, Apex1 and Rgnef were markedly inhibited. By the results above, FAK enhances the malignancy of SGC7901 and SMMC7721 cells, and the expressions of Src, Ras, Apex1 and Rgnef. Our results help people to understand FAK roles as a signal player during the oncogenesis and tumor development under the regulation by complicated signal pathways.

KeyWords:

focal adhesion kinase; gastric cancer; hepatocarcinoma; cell malignancy; gene function