Abstract:
The extraction technology of polyphenols from Polygonatum sibiricum Red. was optimized and its antibacterial activity was evaluated.The single-factor experiment was carried out by using ultrasonic assisted extraction method with ethanol concentration, liquid-solid ratio and ultrasonic time. Then the extraction conditions of polyphenols were optimized by the response surface methodology. The antibacterial activity was evaluated by 96-well plates method to measure minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The results showed that the optimal extraction of polyphenols was as follows: ethanol concentration 62%, liquid-solid ratio 22∶1 mL/g and ultrasonic time 51 min, and the experimentally measured yield of polyphenols was 20.02±0.27 mg/g, with a relative error of only 1.00% to the model-predicted value.The polyphenols was active against Escherichia coli and Bacillus subtilis, with the MIC and MBC was 50 and 100 mg/mL, respectively. The optimization of ultrasonic assisted extraction of polyphenols from Polygonatum sibiricum Red. was reasonable and scientific, and the polyphenols had antibacterial activity.
