自然科学版
陕西师范大学学报(自然科学版)
食品工程与营养科学
气体ClO2对猕猴桃青霉的抑菌作用及其机理
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王烨, 薛敏, 郭晓强, 冯翠娇, 高贵田*
(陕西师范大学 食品工程与营养科学学院, 陕西 西安710119)
高贵田,男,副教授。E-mail:gaoguitian2006@snnu.edu.cn
摘要:
以生长处于对数期的猕猴桃青霉为实验对象,研究气体ClO2对猕猴桃青霉的抑制作用及主要细胞内容物渗漏的影响。结果表明:气体ClO2对青霉孢子的萌发与菌丝体的生长有较好的抑制作用,0.8 mg/L处理7 min及0.6 mg/L处理10 min均可完全抑制猕猴桃青霉孢子的萌发;2.8 mg/L处理25 min可以完全抑制青霉菌丝体的生长。气体ClO2损害了青霉细胞屏障功能及细胞膜的渗透性,气体ClO2对猕猴桃青霉蛋白质、DNA、K+的渗漏量影响较大,与青霉的抑制率相关性较强,可能是抑制青霉的重要原因;而气体ClO2对猕猴桃青霉Mg2+、Ca2+的渗透量影响较小。
关键词:
青霉; 气体ClO2; 猕猴桃; 抑菌机理; 识别分类
收稿日期:
2016-11-14
中图分类号:
TS255.3
文献标识码:
A
文章编号:
1672-4291(2017)06-0115-07
基金项目:
陕西省科技统筹创新工程计划项目(2014KTCL02-19)
Doi:
Bacteriostasis and antimicrobial mechanism of chlorine dioxide gas on penicillium sp. of Kiwi fruit
WANG Ye, XUE Min, GUO Xiaoqiang, FENG Cuijiao, GAO Guitian*
(School of Food Engineering and Nutritional Science, Shaanxi Normal University,Xi′an 710119, Shaanxi, China)
Abstract:
Taking Penicillium sp. of Kiwi Fruit as experimental subjects, the bacteriostasis of chlorine dioxide gas on both Penicillium sp. and the leakage of main cellular content of kiwi fruit are studied. The results shows that chlorine dioxide gas could both inhibit the germination of Penicillium sp. spores and the growth of mycelium. The germination of Penicillium sp. spores were completely inhibited when the kiwi fruit was treated with chlorine dioxide gas about 0.8 mg/L for 7 minutes and about 0.6 mg/L for 10 minutes. And a 25-minute treatment of chlorine dioxide gas under the concentration of 2.8 mg/L might exterminate the growth of mycelium. Chlorine dioxide gas damaged the barrier function and the membrane permeability of Penicillium sp. cells. No matter how long the time of treatment of Penicillium sp. toward kiwi fruit would be, there is always a strong correlation between the leakage of protein, DNA and the inhibition rate of Penicillium sp., which may be the important reason of inhibiting Penicillium sp. Meanwhile, there are minor effects of chlorine dioxide gas on the leakage of magnesium ions and calcium ions.
KeyWords:
Penicillium sp.; chlorine dioxide gas; kiwi fruit; antibacterial mechanism