The selection and primary characterization of a 12mer peptide targeting ovarian cancer cells
SHI Ziqi, XIAO Li, MA Ni, GAO Xiaojie, HAN Juanjuan， LI Jinmei, HOU Yifan, LIU Zheng, XUE Changzhi, SONG Xigui, HOU Yingchun*
(School of Life Sciences, Shaanxi Normal University, Xi′an 710119, Shaanxi, China)
Abstract:
Using SKOV3 as the targeting cells, following the biopanning protocol modified by our lab, after 5 rounds of biopanning we screened out positive phage clones from Ph.D-12 phage displayed peptide library to bind ovarian cancer. Finally using ELISA, cytoimmunofluorescence and other methods, R20 clone was identified as the best clone to target SKOV3 cells with high specificity and sensitivity. The results above will be important primary data for the next study on the reagent or drug delivery system of the targeting diagnosis and therapy of ovarian cancer.
KeyWords:
ovarian cancer; targeting peptides; phage displayed peptide library; molecular imaging diagnosis; targeting therapy
