Abstract:
Complete mitochondrial genomes (mitogenomes) of Diaphaniapers pectalis(Walker, 1859) and Pleuroptya inferior(Hampson, 1898) were sequenced and primarily analyzed by PCR with primer walking. The results showed that the mitogenomes sequence of D. perspectalis and P. inferior were 15 249 bp and 15 348 bp in length. The composition and arrangement of two species mitogenomes were consistent with other sequenced Pyraloidea, and included 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA), two ribosomal RNA (rRNA), and a variable A+T-rich region. All protein-coding genes began with the typical ATN codon except for CO Ⅰ which began with CGA. For stop codon, TAA was used in all the PCGs, while the CO Ⅰ, CO Ⅱ and ND5 existed incomplete stop codon T or TA. All tRNAs had the typical cloverleaf secondary structure except for tRNASer(AGN) which lacks the DHU stem. The A+T-rich region of D. perspectalis and P. inferior was located between the 12S rRNA and tRNAMet, and their lengths were 344 bp and 366 bp, displaying the higher A+T content than 37 genes in the mitogenome. In addition, there are some conserved sequences, poly-T and poly-A structures, as well as different tandem repeats in the A+T-rich regions. Maximum likelihood (ML) and Bayesian inference (BI) phylogenetic analyses were performed based on the 13 PCGs in TenPyraloidea subfamilies show that the results of this study accord with previous morphological and molecular taxonomies of this group.
