Abstract:
The WRKY gene family member SmTTG2 gene had been cloned from Salvia miltiorrhiza by screening transcriptiome data, with Genbank accession number KC161227. SmTTG2 contains a 1 425 bp coding sequence, which encodes a protein of 475-amino acid residues. The calculated molecular mass and predicted isolectric point of the complex protein were 51.28 kDa and 8.58, respectively. And the amino acid seqeucne showed that SmTTG2 belongs to class I subgroup for harbouring 2 WRKY domains and C2H2 motif. In additon, The BLAST search analysis of SmTTG2 showed an highly degree of similarity to the WRKY protein sequences of Cucumis sativus, Tamarix androssowii, and Brassica napus ect. Moreover, the promoter of SmTTG2 gene was cloned and the cis-acting regulatory elements were analyzed through PlantCARE database. The promoter analysis revealed that there are multiple putative cis-acting elements to be involved in response to environmental factors, endosperm development, plant hormones (salicylic acid, MeJA, ethylene, and auxin) responsiveness, anaerobic induction, and some MYB binding sites also found in the promoter of SmTTG2 gene. Quantitative real-time PCR indicated that the SmTTG2 gene is expressed in all tissues tested, however, the highest transcriptional level occurs in flower compared with other tissures including root, stem and leaf. In additon, SmTTG2 is highly expressed during the stages of flowers′ blooming and seeds germination, which indicated that SmTTG2 could be related to seeds devolopment.
