A chemiluminescence sensor for determination of peimisine based on molecularly imprinted polymer as solid phase extraction
HAN Hongping1,4,5, ZHENG Hong2*, MA Tianyi3,LI Baolin1*, YUAN Silan2, TAN Li2， XIANG Hui2
(1 School of Chemistry and Chemical Engineering, Shaanxi Normal University，Xi′an 710119, Shaanxi， China;2 College of Chemistry, Chongqing Normal University, Chongqing 410331, China；3 College of Optoelectronic Engineering, Chongqing University, Chongqing 400044, China;4 School of Chemistry and chemical Engineering, Qinghai Normal University,Xining 810008, Qinghai, China; 5 Key Laboratory of Plant Resources on the Qinghai Tibet Plateau，Xining 810008, Qinghai, China)
Abstract:
The peimisine-imprinted polymer was synthesized by molecular imprinting technique. The selective adsorption performance of the imprinted polymer and non imprinted polymer was investigated. The peimisine-imprinted polymers (IIPs) has higher adsorption capacity and selectivity for peimisine than that of non imprinted polymer. The maximum static adsorption capacity of the imprinted and non-imprinted sorbent for peimisine was 67.59 μmol/g and 21.01 μmol/g, respectively. The methods for the determination of peimisine was based on the enhancement of CL of luminol-H2O2 system. A new molecular imprinting chemiluminescence sensor was established for the determination of peimisine. The CL intensity was linear to peimisine concentration in the range from 1.0×10-5 to 1.5×10-3 mol/L, the detection limit was 0.15×10-5 mol/L, the relative standard deviation was 2.9 %. This method was applied to the determination of the peimisine in Fritillaria cirrhosa D. Don and Fritillaria thunbergii Miq samples with satisfactory results.
KeyWords:
molecular imprinting polymer; chemiluminescence; sensor; peimisine; luminol
