游泳运动改善幼年自发性高血压大鼠腓肠肌微血管稀少的作用及机制-陕西师范大学学报期刊社网站

陕西师范大学学报（自然科学版）

体育科学

游泳运动改善幼年自发性高血压大鼠腓肠肌微血管稀少的作用及机制

张昆茹*, 屈爱静, 杜丽

（陕西师范大学体育学院，陕西西安 710119）

张昆茹,女,教授。E-mail:zhangkunru@snnu.edu.cn

摘要:

通过构建长期有氧运动模型，明确游泳运动对幼年自发性高血压大鼠腓肠肌微血管稀少的作用并探讨其机制。采用4周龄雄性大鼠，分为3组：对照组（WKY）、自发性高血压组（SHR）及高血压运动组（SE）。 HE染色观察腓肠肌微血管密度、微血管数目与肌细胞数目的比值及肌纤维横截面积的变化，运用免疫组化方法和Western Blot方法检测各组大鼠腓肠肌中VEGF、eNOS、 PI3K、Akt的蛋白表达。实验发现:与WKY组相比，SHR组腓肠肌中微血管密度、微血管数目与骨骼肌细胞数目的比值、肌纤维横截面积、腓肠肌总蛋白含量明显减少（P<0.01）；8周游泳训练之后，SE组与SHR组相比，各项指标明显增加（P<0.01）。免疫组化和Western Blot实验结果显示：与WKY组相比， SHR腓肠肌中的VEGF、PI3K、Akt、eNOS表达明显下降，SE组腓肠肌中 VEGF、PI3K、Akt、eNOS蛋白表达与SHR相比均有明显增加；游泳运动增加了SHR 腓肠肌中VEGF 、PI3K、Akt、 eNOS的蛋白表达。有氧运动可以改善幼年自发性高血压大鼠腓肠肌中微细血管稀少的现象，其机制与运动增加VEGF及eNOS的表达及上调PI3K/Akt信号通路有关。

关键词：

自发性高血压; 游泳运动; 血管内皮生长因子; 微血管稀少

收稿日期：

2016-11-20

中图分类号：

G804.4

文献标识码：

文章编号：

1672-4291(2017)03-0119-06doi:10.15983/j.cnki.jsnu.2017.03.235

基金项目：

国家自然科学基金（31071035）;陕西省自然科学基金(2016JM3008)

Doi:

Swimming exercise improve microvascul rarefaction of gastrocnemius in young spontaneously hypertensive rats and the underlying mechanisms

ZHANG Kunru*, QU Aijing, DU Li

(School of Physical Education, Shaanxi Normal University, Xi′an 710119, Shaanxi, China)

Abstract:

The aims of this study were to determine the effects of swimming exercise on microvascular rarefaction of gastrocnemius in young spontaneously hypertensive rats and to investigate the underlying mechanisms. 4-week-old male rats were divided in to 3 groups: WKY (Wistar Kyoto group), SHR(spontaneously hypertensive rats group) , SE（spontaneously hypertensive exercise rats group）, SE received 8-week freeloading swimming exercise (1.5 h/day, 5 d/week). Microvascular density,the ratio of the microvascular number to fiber number ,fiber cross sectional area,total protein content in gastrocnemius and the expressions of VEGF,PI3K,Akt and eNOS in gastrocnemius were determined at the end of exercise.The results show that compared with WKY,microvascular density,the ratio of the microvascular number to fiber number,fiber cross sectional area and total protein content in gastrocnemius tissue were decreased in SHR.Compared with SHR（P

KeyWords:

spontaneously hypertension； swimming exercise； VEGF； microvascular rarefaction