Abstract:
A main component HEP, extracted from the leaves of epimedium, is a protein bound polysaccharide which identified by its physical and chemical properties. The total sugar mass fraction of HEP is 89.5%, the content of galactose acid is 10.26%, and the protein content is 4.39%. A chronic fatigue model was established by using multiple induced factors: the constraint, running platform, and congested and noisy environment. Giving certain amount of HEP, through the observation of rats' weight, intake of food and drinking water quantity, combining with general scoring scale and behavioral science experiment(water maze, open field, hanging tail test), the HEP's impact on CFS model was evaluated. Finally, the urinary of chronic fatigue rats was detected by GC-MS Technology of metabolism. And the difference of each group′s metabolite and metabolic pathway were found out .The results showed that the urine metabolites of the 3 groups were well differentiated. A total of 11 metabolites were obtained between the CFS model and control groups with 5 potential metabolic biomarkers. And HEP mainly effects on chronic fatigue rats through two pathways: the disorder of HPA and the metabolism of arginine and proline.
