自然科学版
陕西师范大学学报(自然科学版)
生命科学
HPLC法测定不同季节成熟的树莓中酚类化合物、花色苷及VC的差异性
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李敏1, 尚宏丽2*, 李亮1, 李丽洋2, 张玉莹2
(1 锦州医科大学 食品科学与工程学院;2 辽宁省肉类加工与质量安全控制工程技术研究中心, 辽宁 锦州 121001)
尚宏丽,女,副教授。E-mail:shanghongli007@126.com
摘要:
研究两种不同季节成熟的树莓:海尔特兹(Heritage)、秋福(Autumn Bliss)、费尔杜德(Fertod Zamatos)、米克(Meeker)共4个品种中酚类化合物、花色苷以及维生素C(VC)的含量并作分析比较。用高效液相色谱法测定两种不同季节成熟的4种树莓中酚类物质及6种单体酚含量,同时测定花色苷总含量及维生素C的含量并做分析比较。两个季节成熟的4个品种树莓中总多酚的含量由高到低的次序为:海尔特兹>秋福>费尔杜德>米克。其中单体酚的种类均为鞣花酸、阿魏酸、咖啡酸、对香豆酸、对羟基苯甲酸、槲皮素。4个品种的树莓中,海尔特兹的花色苷及维生素C的含量明显高于其他品种。树莓具有良好的抗氧化性,本研究中的4个品种中,秋果型双季莓海尔特兹与其他3种品种相比含有较高的植物化学物质(酚类化合物、VC、花色苷)的水平,可以利用其生产出功能性更好,附加性更高的树莓新品种。
关键词:
高效液相色谱法; 酚类化合物; 花色苷; 维生素C; 树莓
收稿日期:
2016-03-25
中图分类号:
TS255
文献标识码:
A
文章编号:
1672-4291(2017)02-0075-05doi:10.15983/j.cnki.jsnu.2017.02.126
基金项目:
辽宁省自然科学基金(2015020776)
Doi:
Determination of phenolic compounds,anthocyanins and VC from ripe raspberries in different seasons using high performance liquid chromatography
LI Min1, SHANG Hongli2* , LI Liang1, LI Liyang2, ZHANG Yuying2
(1 College of Food Science and Engineering, Jinzhou Medical University;2 Liaoning Meat Processing and Quality Safety Control Center, Jinzhou 121001, Liaoning, China)
Abstract:
The contents of phenols including 6 phenol monomers ,anthocyanin and VC of four species of ripe raspberries in different season using HPLC.Raspberries of “Heritage” was found to contain the highest phenol content followed by “Autumn Bliss”, “Fertod Zamatos” and “Meeker”.Different types of polyphenol monomers were found in the studied varieties of raspberries and ellagic acid,caffeic acid, ferulic acid, pcoumaricacid, catechin and “quercetin” were simultaneously present in them.Heritage of anthocyanins and VC content is significantly higher than the other varieties of raspberries.The present study outlines that the raspberry is an extremely rich source of antioxidants. Bioactive compounds in heritage raspberries was very obvious,further studies should be conducted among native populations, in order to determine those high in antioxidant properties, which could help for the selection and validation of the most productive cultivars.
KeyWords:
HPLC; anthocyanins; polyphenols; VC; raspberry