Investigation of interaction between DNAzyme and polyvinylpyrrolidone(PVP)/Silica/Ru(bpy)2+3 nanoparticles for label-free electrogenerated chemiluminescent detecting Pb(Ⅱ)
FU Wen， CAI Linfang， GUO Zhihui, ZHENG Xingwang*
(Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi′an 710119, Shaanxi, China)
Abstract:
The PVP/Silica/Ru (bpy)2+3 nanoparticles were synthesized, which show different affinities for single and double-stranded DNA.It was found that changes of DNAzyme structure induced by Pb(Ⅱ) resulted in the change in electrochemiluminesence properties of nanoparticles in the Nafion/carbon nanotubes modified electrode. Based on this finding, a new electrochemiluminesence method for detecting Pb(Ⅱ) was developed.The method is a label-free DNA probe, no immobilized DNAzyme, and high sensitivity, etc. Under the optimal experimental conditions, this new electrochemiluminescence approach for Pb(Ⅱ) presented a detection limit of 0.5 nmol/L, and was successfully applied for detecting Pb(Ⅱ) in water samples.
KeyWords:
electrochemiluminescence; DNAzyme; silica nanoparticles; Ru(bpy)2+3; Pb(Ⅱ)
