反相高效液相色谱测定太白泡沙参中β-谷甾醇的含量-陕西师范大学学报期刊社网站

陕西师范大学学报（自然科学版）

化学与材料科学

反相高效液相色谱测定太白泡沙参中β-谷甾醇的含量

刘娟1，赵秋艳1，李鹏程2，陈战国2*

（1 西安交通大学第二附属医院，陕西西安 710004;2 陕西师范大学化学化工学院，陕西西安 710119）

陈战国，男，副教授，博士。E-mail: chzhg@snnu.edu.cn

摘要:

建立了反相高效液相色谱(RP-HPLC)测定太白泡沙参中β-谷甾醇含量的方法。在色谱柱为Kromasil C18 柱(5 μm，4.6 mm×250 mm)、紫外检测（波长210 nm）、色谱柱温度35 ℃，以甲醇为流动相且流速为0.7 mL/min的优化条件下，β-谷甾醇在 4×10-7～2×10-5g范围内线性关系良好，r=0.999 9，平均回收率为 99.70%，测定太白泡沙参中β-谷甾醇平均含量为0.134% 。此分析方法简便准确，可作为该产品的质量控制方法。

关键词：

太白泡沙参； β-谷甾醇；反相-高效液相色谱

收稿日期：

2016-08-26

中图分类号：

R284.1

文献标识码：

文章编号：

1672-4291(2017)01-0061-03doi:10.15983/j.cnki.jsnu.2017.01.313

基金项目：

国家自然科学基金（20572066); 陕西省自然科学基金(2009JM2001)

Doi:

RP-HPLC determination of β-sitosterol in A.potaninii Korsh from Shaanxi Taibai Mountainous area

LIU Juan1, ZHAO Qiuyan1, LI Pengcheng2, CHEN Zhanguo2*

(1 The Second Affiliated Hospital of Xi′an Jiaotong University, Xi′an 710004, Shaanxi, China;2 School of Chemistry and Chemical Engineering, Shaanxi Normal University,Xi′an 710119, Shaanxi, China）

Abstract:

A method for the determination of β-sitosterol in A.potaninii Korsh from Shaanxi Taibai Mountainous area by reverse phase-high performance liquid chromatography(RP-HPLC) has been developed. The Kromasil C18 column (4.6 mm×250 mm，5 μm) was used for the separation with a mobile phase of pure methanol. The detected wavelength was 210 nm with a UV detector. The flow rate of mobile phase was 0.7 mL/min and the column temperature was 35 ℃ for the accurate determination of β-sitosterol. The method has a good linearity within a range of 4.0×10-7~2.0×10-5 g(r=0.999 9) under optimized conditions. The average recovery was 99.70%. The content of β-sitosterol in A.potaninii Korsh from Shaanxi Taibai Mountainous area was determined, and the average content of β-sitosterol was 0.134%. This method is simple and accurate to be used for the evaluating quality of A.potaninii Korsh.

KeyWords:

Taibai A.potaninii Korsh; β-sitosterol; RP-HPLC