Abstract:
In order to obtain staphylococcus aureus enterotoxin B (SEB) efficintly for the research of rapid detection of SEB, SEB was expressed by prokaryotic expression system, the purification method of the recombinant SEB was studied, and its antigenicity was identified by Western blot and the magnetic immunochromatographic rapid assay strip for detecting SEB. Studies showed that SEB was effective expression in E.coli BL21(DE3) by SDS-PAGE detection, and the 65．8% of special expressed product in total somatic proteins, and the product with purity of 95.2% could be obtained by using two-step purifications including Ni affinity chromatography and DEAE anion exchange chromatography. The expression product bound specifically to McAb against SEB was proved by Western blot and the magnetic immunochromatographic rapid assay strip for detecting SEB, indication that the recombinant SEB can be used in quick detection strip of SEB.
