厚皮甜瓜贮期腐烂病病原菌分离鉴定及抑菌实验研究-陕西师范大学学报期刊社网站

陕西师范大学学报（自然科学版）

食品工程与营养科学

厚皮甜瓜贮期腐烂病病原菌分离鉴定及抑菌实验研究

吴倩1，张润光1*，张亮2，江琦1，张有林1

（1 陕西师范大学食品工程与营养科学学院, 2 生命科学学院，陕西西安 710119）

张润光，男，讲师。E-mail: sunshine@snnu.edu.cn

摘要:

对厚皮甜瓜采后贮藏期间腐烂病的病原菌进行了分离、纯化与鉴定，研究了ε-聚赖氨酸、壳寡糖、纳他霉素和右旋龙脑对厚皮甜瓜病原菌生长繁殖的影响。结果表明，厚皮甜瓜贮期腐烂病病原菌为链格孢菌(Alternaria alternata)和爪甲曲霉(Aspergillus unguis)。ε-聚赖氨酸和纳他霉素抑菌效果显著，当ε-聚赖氨酸浓度为20.0 mg/L、纳他霉素浓度为4.0 mg/L时，即可完全抑制两种病原菌的生长繁殖。壳寡糖和右旋龙脑对链格孢菌和爪甲曲霉均有一定的抑制作用。

关键词：

厚皮甜瓜；贮期病原菌；分离；鉴定；抑菌

收稿日期：

2014-07-15

中图分类号：

TS255.3

文献标识码：

文章编号：

1672-4291(2016)05-0119-06doi:10.15983/j.cnki.jsnu.2016.05.454

基金项目：

陕西省科技统筹创新工程计划（2013KTZB02-03-03）；西安市农业应用技术研究项目（NC10017）

Doi:

Study on separation and identification of rot pathogens for postharvest Cucumis melo L. during storage and its antimicrobial experiments

WU Qian1, ZHANG Runguang1*, ZHANG Liang2, JIANG Qi1, ZHANG Youlin1

(1 School of Food Engineering and Nutritional Science,2 School of Life Sciences, Shaanxi Normal University, Xi′an 710119, Shaanxi, China)

Abstract:

The pathogens causing the rot of Cucumis melo L. fruit during storage were separated and identified.And effects of the ε-polvlvsin, chitosan, natamycin and borneol on the reproduction of rot pathogens were studied. The results showed that the rot pathogens were Alternaria alternate and Aspergillus unguis.The suppressive effect of ε-polvlvsin and natamycin on the pathogens were remarkable. When the concentration of ε-polvlvsin was 20.0 mg/L and the concentration of natamycin was 4.0 mg/L, the reproduction of these two pathogens could be inhibited completely. Chitosan and borneol both had a certain inhibitory effect on Alternaria alternate and Aspergillus unguis.

KeyWords:

Cucumis melo L.; pathogen; purification; identification; antimicrobial