大金发藓乙酸乙酯提取物对K562细胞膜的损伤效应-陕西师范大学学报期刊社网站

陕西师范大学学报（自然科学版）

生命科学

大金发藓乙酸乙酯提取物对K562细胞膜的损伤效应

郭玲1，雷成康2，袁文娟1，汤薇3，王攀1，刘怡琳1，王筱冰1*

（1 陕西师范大学生命科学学院，陕西西安 710119； 2 西安市食品药品检验所，陕西西安 710054；3 陕西师范大学化学化工学院，陕西西安 710119）

王筱冰，女，副教授，博士。E-mail：wangxiaobing@snnu.edu.cn

摘要:

为了探讨大金发藓乙酸乙酯提取物（PC-EEF）对K562细胞的膜损伤效应,应用MTT法检测PC-EEF对多种肿瘤细胞的细胞毒活性,台盼蓝拒染实验检测细胞存活力,相差显微镜观察K562细胞形态的变化,FDA-PI双染检测细胞膜通透性变化,DiBAC4 (3)染色检测细胞膜电位变化,Hoechst 33342-PI双染进一步验证细胞死亡的发生。研究结果显示:PC-EEF能显著地杀伤多种肿瘤细胞，其中对K562细胞最为敏感，PC-EEF以时间和剂量依赖性的方式降低 K562细胞存活力。PC-EEF可诱导K562细胞发生显著膜损伤效应，表现为膜形态改变、膜通透性增加和膜电位下降。

关键词：

大金发藓；乙酸乙酯组分； K562细胞；细胞毒活性；膜损伤效应

收稿日期：

2016-01-06

中图分类号：

R285.5; R73-3

文献标识码：

文章编号：

1672-4291(2016)05-0089-05doi:10.15983/j.cnki.jsnu.2016.05.355

基金项目：

国家自然科学基金（81202957）；陕西省自然科学基金（2014JQ4144）

Doi:

Damage effect of ethyl acetate extract of Polytrichum commune on membrane of human leukemia K562 cell

GUO Ling1， LEI Chengkang2， YUAN Wenjuan1， TANG Wei3，WANG Pan1， LIU Yilin1， WANG Xiaobing1*

（1 School of Life Sciences，Shaanxi Normal University，Xi′an 710119， Shaanxi, China;2 Xi′an Institute for Food and Drug Control，Xi′an 710054， Shaanxi, China;3 School of Chemistry & Chemical Engineering，Shaanxi Normal University，Xi′an 710119， Shaanxi, China）

Abstract:

The effect of ethyl acctate extract of Polytrichum commune L.ex Hedw on the membrane of human leukemia K562 cell was investigated. MTT assay was used to evaluate the cytotoxicity of PC-EEF on various cancer cells. Trypan blue exclusion test was also adopted to assess the viability of K562 cells. Cell morphological changes were evaluated under phase-contrast microscopy. Changes on plasma membrane integrity and cell membrane potential were analyzed by FDA-PI double fluorescent dye staining and DiBAC4 (3) staining，respectively. Hoechst 33342-PI double staining further confirmed the occurrence of cell death. Results showed that PC-EEF could effectively damage several cancer cells and K562 cell was the most affected one. PC-EEF decreased K562 cell viability in a dose-and time-dependent manner. PC-EEF exerted significant cell membrane damage effects，including cell morphological deformation，membrane integrity loss and membrane depolarization.

KeyWords:

Polytrichum commune L.ex Hedw； ethyl acetate extract； K562 cell; cytotoxic activity； cell membrane damage effect