花鼠尿液中挥发性成分分析-陕西师范大学学报期刊社网站

陕西师范大学学报（自然科学版）

生命科学

花鼠尿液中挥发性成分分析

王瑶1，张博1，周宏凤1，韩崇选2，李金钢1*

(1 陕西师范大学生命科学学院，陕西西安 710119；2 西北农林科技大学林学院，陕西杨凌 712100)

李金钢，男，教授，博士生导师。E-mail: jingang@snnu.edu.cn

摘要:

利用顶空固相微萃取-气相色谱-质谱联用技术 (HS-SPME-GC/MS) 对动情期不同性别花鼠尿液中的化学成分进行检测，初步分析出其中35种化学物质，主要为酯类、醇类、酚类、酮类、醛类、含氮化合物、含硫化合物以及烯烃类物质等。结果表明，这些物质基本为雌雄共有，但各物质的含量却有所不同。其中，2,5-二甲基吡嗪(2,5-Dimethylpyrazine)、4-甲基苯酚 (4-Methylphenol) 、4-乙基苯酚 (4-Ethylphenol) 有显著的性别差异。据此推测这3种物质可能是花鼠的性信息素成分。

关键词：

花鼠；尿液；挥发性成分；信息素

收稿日期：

2015-12-16

中图分类号：

Q955

文献标识码：

文章编号：

1672-4291(2016)05-0082-07doi:10.15983/j.cnki.jsnu.2016.05.354

基金项目：

国家林业公益性行业科研专项经费(201404405-02)

Doi:

Analysis of volatile compounds in urine of Eutamias sibiricus

WANG Yao1， ZHANG Bo1， ZHOU Hongfeng1， HAN Chongxuan2， LI Jingang1*

(1 School of Life Sciences, Shaanxi Normal University, Xi′an 710119, Shaanxi, China; 2 School of Forestry, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, Shaanxi, China)

Abstract:

Headspace-solid phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC/MS) were used to analyze the composition of estrous urine of male and female Eutamias sibiricus, 35 of them were putatively identified，and mainly contained esters, alcohols, phenols, ketones, aldehydes, nitrogen compounds, sulfur compounds, and olefins substances, etc. The results showed that all the compounds detected were shared by males and females, but the levels of each one was different. Among them, 2,5-Dimethylpyrazine, 4-Methylphenol, 4-Ethylphenol showed significant sexual difference. Thus, these compounds might be pheromone in Eutamias sibiricus, and play some roles in sex attractiveness.

KeyWords:

Eutamias sibiricus; urine; volatile compounds; pheromone