The primers at C1 and Te region of NRPS gene were designed respectively and PCR technology was employed to find Pseudomonas fluorescens carrying cyclic lipopeptide genes from soil samples of Fujian and Shaanxi province.Then SSCP and Restriction endonuclease method were carried out to analyze the diversity of NRPS genes.Results show that the C1 and Te regions of Fujian and Shaanxi bacterial strains display 3,5,7 three kind bands by SSCP electrophoresis and the positions of bands are different. C1 region has 10 types,and Te region has 10 types in total. Among them Fujian strains have 9 types except SSCP-10,while Shaanxi strains have 8 types except SSCP-4 and SSCP-2.Cutting Te region of NRPS genes by two different restriction enzymes HinfⅠ and SphⅠ,the digestion products of Fujian strains are divided into 15 kinds,while Shaanxi strains are divided into 11 kinds.It shows that NRPS gene has a variety of forms, and the types of distribution in different regions vary.In conclusion,the NRPS genes in Fujian strains have more diversity which suggests that these strains have more potential to produce new types of lipopeptide.

Pseudomonas fluorescens; cyclic lipopeptid; NRPS gene; polymorphism