JIANG Yan, ZHANG Quanxing, GUAN Delong, XU Shengquan*
（Co-Innovation Center for Qinba Regions Sustainable Development，College of Life sciences,Shaanxi Normal University, Xi′an 710119, Shaanxi, China）
Abstract:
The codon bias of Hynobiidae Cytochrome b genes among 16 species were analyzed by using the software Mobyle online tool CUSP program, CodonW, and Cluster3.0, and a further discussion on the Codon bias effection on phylogenetic analysis was done. The results showed that the ENC（effective number of codons） of these 16 Hynobiidae Cytb genes range from 34.45 to 47.18, with average value at 41.01, which shown a preference on codon bias. The nucleotide base composition analysis at each locus showed Hynobiidae Cytochrome b prefers to codes end by A or T bases. The hierarchical clustering of RSCU values and species showed obvious codon usage patterns among genera. The clustering results slightly deviated from the Maximum-likelihood and the Bayes phylogenetic trees.
KeyWords:
Hynobiidae animals; Cytb gene; codon bias; phylogeny
