自然科学版
陕西师范大学学报(自然科学版)
生命科学
聚乙烯醇-海藻酸钠-活性炭固定化大肠杆菌在不同水体中的毒性检测
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徐颖超, 刘畅*, 常晓杰
(锦州医科大学 药学院, 辽宁 锦州 121000)
刘畅,女,副教授,博士。E-mail:liuchang_198006@163.com
摘要:
为构建以铁氰化钾为探针的电化学用于化学品的毒性检测方法,初步探讨了聚乙烯醇(PVA)与添加剂海藻酸钠(SA)、活性炭(AC)共固定包埋大肠杆菌(E. coli)的活性微生物颗粒在实验室废水、毒物3,5-二氯苯酚(DCP)、杀虫剂乙嘧酚以及除草剂五氰磺草胺的水样毒性分析中的应用性能。结果表明:添加剂SA和AC的质量分数均为1% ,交联剂CaCl2的质量分数为2%为本研究最优实验条件,此时所测毒物对微生物固定化颗粒的呼吸抑制作用依次为DCP>乙嘧酚>五氰磺草胺>实验室废水,抑制率分别是30.23%~55.35%、25.14%~46.23%、20.12%~38.33%和5.74%~27.89%,抑制率和灵敏度均明显优于仅由PVA-SA单独固定包埋的大肠杆菌颗粒。
关键词:
添加剂; 微生物固定化; 电化学; 毒性检测
收稿日期:
2015-06-25
中图分类号:
X522
文献标识码:
A
文章编号:
1672-4291(2016)03-0085-06doi:10.15983/j.cnki.jsnu.2016.03.335
基金项目:
辽宁省自然科学基金(2014022037); 辽宁医学院博士启动基金(Y2012B006)
Doi:
Application of polyvinyl alcohol-alginate-activated carbon immobilized E. coli in testing toxicity of different wastewater
XU Yingchao, LIU Chang*, CHANG Xiaojie
(College of Pharmacy,Jinzhou Medical University,Jinzhou 121000,Liaoning, China)
Abstract:
To construct electrochemical detection toxicity of chemicals in water using ferricyanide as a probe, the toxicity of chemicals in different wastewater including laboratory wastewater, and poison of 3,5-dichlorophenol (DCP), pesticide ethirimol, herbicide five cyanide alachlor were determined by polyvinyl alcohol-alginate-activated carbon immobilized E. coli. The following optimal experimental conditions were obtained.The quality score of sodium alginate and activated carbon additive are 1%, amount of cross-linking agent CaCl2 is 2%. The order in the respiratory depression effect of toxicants on the microorganisms immobilized on particle are as following, DCP > ethirimol > five cyanide alachlor > laboratory waste, inhibition rate range of 30.23%~55.35%, 25.14%~46.23%, 20.12 %~38.33%~27.89% and 5.74%, respectively, and the sensitivity was significantly better than that treated by the PVA-SA immobilized E.coli.
KeyWords:
addition agent; immobilized microorganism; electrochemistry; toxicity testing