In order to select bacteria with producing serine protease for biological control, BApNA, the particular substrate of serine protease and selective inhibitor PMSF were used to confirm serine protease productivity. Morphological characteristics, physiological, biochemical characteristics and 16s rDNA comparison analysis were used for strain identification. Antagonistic method, inhibition zone method and liquid shake culture were introduced to observe the resistant effect of the bacteria on plant pathogenic fungi. 96-well plate assay was used to observe the resistant effect the bacteria on nematodes. SNUB19, a bacillus with producing serine protease was screened in the experiment and was identified as Bacillus subtilis. The fermentation broth of this strain is significantly resistant to plant pathogenic fungi and is relatively fatal to Meloidogyne incognita.

serine protease; bacillus; strain classification; plant pathogenic fungi; Meloidogyne incognita