ZHU Chengang1,2, GUO Mingxiong2, SHAO Huanjie3*
（1 School of Chemistry and Life Sciences, Guizhou Normal College, Guiyang 550018，Guizhou，China；2 College of Life Sciences, Wuhan University, Wuhan 430072， Hubei, China；3 School of Life Sciences, Shaanxi Normal University, Xi′an 710119， Shaanxi, China）
Abstract:
In order to study the function of ZNF268 gene in tumorigenesis, five solid tumor specimens were collected and analyzed, which were compared to 17 peripheral blood specimens of healthy donors, by reverse transcription-polymerase chain reaction (RT-PCR), nested PCR and sequencing. Results demonstrated that the patterns of alternative splicing of ZNF268 gene in human solid tumors were dramatically different from healthy blood. The probability of appearance of ZNF268a was 100% in healthy peripheral blood, which was 0 in solid tumors. These results suggested that the disappearance of ZNF268a may contribute to cancerization and is a potential prognostic factor.
KeyWords:
ZNF268 gene; aberrant alternative splicing; solid tumor; biomarker
