Poly(thymine)-templated copper nanoparticles as fluorescent probes for highly sensitive detection of L-cysteine
WU Jia, LIU Wei, LI Baoxin*
(Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi′an 710119, Shaanxi, China)
Abstract:
A simple and sensitive method for the detection of L-cysteine was established using fluorescent copper nanoparticles (CuNPs) synthesized by polythymine (poly T) as a templet. In the presence of L-cysteine, the fluorescence of CuNPs was tremendously quenched through a robust Cu-S bond between -SH in cysteine molecule and Cu of CuNPs. Under the optimized experimental conditions, the probe exhibited excellent performance in the range from 3 to 50 μmol/L with a detection limit of 0.56 μmol/L. Moreover, the developed bioassay was successfully applied to selective detection and determination of L-cysteine in human urine samples.
KeyWords:
polythymine; copper nanoparticles; fluorescence; L-cysteine
