自然科学版
陕西师范大学学报(自然科学版)
体育科学
游泳对攻击行为大鼠肾脏形态及HSP70表达的影响
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胡柏平1, 汪晓瑞1, 夏敏慧2*
(1 陕西师范大学 体育学院, 陕西 西安 710119; 2 海南师范大学 体育学院, 海南 海口 571158)
胡柏平,男,教授,研究方向为运动生理学。E-mail:hubp166@snnu.edu.cn
摘要:
探讨游泳运动对攻击行为大鼠肾脏形态及HSP70表达的影响。健康雄性SD大鼠42只,随机分为5组:安静对照组(A组,n=8),群居游泳组(B组,n=8),攻击对照组(C组,n=8),攻击游泳组(D组,n=8),入侵组(E组,n=10)。采用单笼饲养加入侵鼠攻击建立攻击模型,建模成功后,群居游泳组和攻击游泳组按照训练要求进行8周的游泳训练。取材制作大鼠肾脏切片,进行HE染色,采用免疫组织化学染色和Western Blot方法观察并分析大鼠肾脏HSP70的表达。与安静对照组相比,攻击对照组及攻击游泳组大鼠肾脏均存在不同程度的形态学改变,两者相比攻击对照组改变明显;群居游泳组大鼠肾脏HSP70蛋白表达显著低于安静对照组(P<0.01),攻击对照组表达显著高于安静对照组(P<0.01),攻击游泳组表达显著低于攻击对照组(P<0.01)。双因素方差分析显示,游泳运动和攻击性应激的交互作用对大鼠肾脏HSP70蛋白的表达产生显著影响。中等强度游泳运动可以拮抗攻击行为大鼠肾脏过强的应激反应并减少形态学改变。
关键词:
游泳;攻击行为;HSP70
收稿日期:
2015-01-22
中图分类号:
G804.2
文献标识码:
A
文章编号:
1672-4291(2015)03-0104-05doi:10.15983/j.cnki.jsnu.2015.03.235
基金项目:
陕西师范大学“211工程”学科建设项目(2014)
Doi:
Effect of swimming on kidney morphology and expression of HSP70 in rats with aggressive behavior
HU Baiping1, WANG Xiaorui1, XIA Minhui2*
(1 School of Physical Education, Shaanxi Normal University, Xi′an 710119, Shaanxi, China; 2 School of Physical Education, Hainan Normal University, Haikou 571158, Hainan, China)
Abstract:
The purpose of this study was to investigate the effect of swimming on kidney morphology and expression of HSP70 of rats with aggressive behavior.42 healthy male SD rats were randomly divided into following 5 groups after training, which are:quiet control group (group A, n=8), communal swimming group (group B,n=8),aggressive behavior control group (group C, n=8), aggressive behavior and swimming group(group D,n=8) and invasion group (group E,n=10).The attack model is established by the ways of feeding the rats separately and let rats attack.Then the rats in the group B and group D were required to have swimming training with 8 weeks. Moreover, made slices of rats′ kidneies and HE stain were made after drawing materials. The rats′ kidneys morphology and expression of HSP70 were measured by Western blotting and immunohistochemical staining.The rats′ kidneys in group C and group D had many morphological changes,especially in group C.The protein of HSP70 of rats′ kidneys in group B is much less than in group A(P
KeyWords:
swimming; aggressive behavior; HSP70