LI Weiling, ZHANG Yaling, LI Yanqing, ZHANG Lijuan, XIA Haibin*
(School of Life Sciences,Co-Innovation Center for Qinba Regions′ Sustainable Development， Shaanxi Normal University , Xi′an 710119, Shaanxi, China)
Abstract:
To produce monoclonal antibodies against human Grn E, GST-Grn E fusion protein was expressed using the prokaryotic expression system, then it was used to immune Balb/c mice. Finally, five hybridoma cell lines secreting Mabs against Grn E were obtained by limited clone dilution. Three hybridomas (1D5, 2E1 and 3F7) were selected to prepare ascites, then further identified by the western blot and immunofluorescence staining. In addition, 1D5 and 3F7 were selected for doing immunoprecipitation. The experimental results indicated that the monoclonal antibodies raised in the paper are specific against human Grn E. The success of the preparation of Mabs against Grn E provides an important tool to the study of PGRN function and its molecular mechanisms.
KeyWords:
progranulin; monoclonal antibody; Grn E; hybridoma
