木瓜提取液对大鼠骨组织中ColⅠ和Cath L表达的影响-陕西师范大学学报期刊社网站

陕西师范大学学报（自然科学版）

生命科学

木瓜提取液对大鼠骨组织中ColⅠ和Cath L表达的影响

李旭斌，陈秀峰，王桂敏 *，艾浩

（1 辽宁医学院研究生院，辽宁锦州 121001； 2 航天中心医院普外科，北京 100049; 3 辽宁医学院第一附属医院，辽宁锦州 121001）

李旭斌，男，硕士研究生，研究方向为生物化学及中西结合抗衰老.Email:15910624691@163.com.

摘要:

将50只SD雌性大鼠随机分为5组，连续给木瓜提取液12周后，测定大鼠Ⅰ型胶原( Col Ⅰ)、组织蛋白酶L( Cath L)、Ⅰ型前胶原氨基端前肽(PINP)、Ⅰ型胶原交联羧基末端肽( ICTP)并行股骨并进行形态学分析.结果表明：木瓜提取液组与雌激素组能显著增加大鼠的骨小梁的数量、降低Cath L的表达，木瓜高剂量组与雌激素组无明显差异(P＞0.05)，这一作用在高剂量组更加明显.木瓜提取液能够降低破骨细胞Cath L 的表达来抑制骨基质中Col Ⅰ的分解，从而起到抗骨质疏松的疗效和骨保护作用.

关键词：

木瓜；骨质疏松； Ⅰ型胶原；组织蛋白酶L； Ⅰ型前胶原氨基端前肽； Ⅰ型胶原交联羧基末端肽

收稿日期：

2013-04-17

中图分类号：

R285

文献标识码：

文章编号：

1672-4291(2014)05007504

基金项目：

国家自然科学基金资助项目（81173443）.

Doi:

The effects of papaya extract on the expression of Col Ⅰ and Cath L in the bone tissue of rats

LI Xubin　,　, CHEN Xiufeng　, WANG Guimin　*, AI Hao1

1 Graduate School of Liaoning Medical University，Jinzhou 121001, Liaoning,China；2 Department of General Surgery Aerospace Tenter Hospital, Bejing 100049, China;3 First Affiliated Hospital, Liaoning Medical University, Jinzhou 121001, Liaoning, China )

Abstract:

50 SD female rats were divided in to 5 groups in a random way, continusly treated with papaya for 12 week, and the Rat Collagen Ⅰ(ColⅠ), Cathepsin L(Cath L), procollagen INterminal propeptide (PINP), carboxyterminal telopeptide of typeⅠcollagen (ICTP) and morphology were determined.The following results obtained.The rats in group of papaya and group of estrogen obviously increased in number of bony trabecule , decreased in the Cath L expression. There were no difference between group of papaya with high does and group of estrogen (P＞0．05),and it was more effective in high dose group.The results imply that the high does papaya can be able to decrease the Cath L expression of osteoclast, consequently inhibited the dissolve of Col Ⅰ, result in protection of bone and antiosteoporosis

KeyWords:

papaya; osteoporosis; Collagen Ⅰ; Cathepsin L；procollagen INterminal propeptide(PINP); carboxyterminal telopeptide of type Ⅰ collagen(ICTP)