SRAPPCR amplification system on Akebia trifoliate was optimized by the orthogonal design of L16（45）,The optimal PCR system was obtained as 1×Reaction Buffer, 2.5 mmol/L Mg2+, 0.2 mmol/L dNTPs, 2 U TaqDNA polymerase, 0.4μmol/L primer, 20 ng genomic DNA in 25 μL reaction system. 6 primer pairs from 100 primer combinations were selected to analyze the genetic diversity relationship of 62 strains Akebia trifoliate from Qinling region. There were 142 clear bands produced, the rate of polymorphism was 87.32%. The result of cluster analysis based on SRAP markers showed that 62 stains Akebia trifoliate could be clustered into 3 groups at 0.72 of genetic similarity coefficient. Every individual of one population was clustered in the same group. It is consistant with the sample collecting zone in group. Therefore, the SRAP system can provide abundant information site, which can be used in the research of genetic diversity of Akebia trifoliate in future.

Akebia trifoliate; Sequencerelated amplified polymorphism(SRAP); system optimization; genetic diversity