Study on separation of azadirachtin from new neem kernels by orthogonal design mode and aqueous two-phase system
WANG Man, WANG Jiajing, LUAN Long, SUN Runguang*
(College of Physics and Information Technology，Shaanxi Normal University， Xi′an 710119，Shaanxi, China)
Abstract:
The separation of azadirachtin from new neem kernels was studied by alcohol- inorganic salt aqueous two-phase extraction technology.The single factor and orthogonal experiments were done to investigate the varitaion of azadirachtin extraction ratio relating to inorganic salt concentration,extraction time and material-solvent ratio. The results showed that the extraction rate of azadirachtin was 1.23% under the optimum condition that the concentration of inorganic salt was 0.62 g/mL, the extraction time was 1.5 h, and the material-solvent ratio was 1∶10 (g∶mL).
KeyWords:
purifying by aqueous two-phase; extraction rate; azadirachtin
