The selection and characterization of the peptides binding to the cells and tissues of gastric carcinoma
LI Rui, WANG Han, MA Caixia, GUO Yonge, WANG Qixuan, XU Jinhui, WU Qiong, XU Nan, HOU Yingchun*
(College of Life Science, Shaanxi Normal University, Xi′an 710119, Shaanxi, China)
Abstract:
Using phage displayed peptides library screening method, we previously screened out two peptides specifically binding to gastric carcinoma cell SGC-7901. The specific affinity of GSP5 clone was confirmed as best using immunofluorescence assays. Then, the specific affinities of GSP5 clone to the cells and tissues of gastric cancer were further confirmed using different immunofluorescence assays under different microscopes.The results show that the peptide clone GSP5 binds to the cells and clinical tissues of gastric cancer at high affinity. Therefore, the clone GSP5 is of the potential to be used as a peptide probe for the early diagnosis and targeting therapy of gastric cancer.
KeyWords:
peptide probe; peptide screening; gastric cancer; phage displayed peptide library
