WANG Yuan1, SU Xiaomin1, LIU Jing2, ZHANG Ting1, WANG Xiaobing1*, LIU Quanhong1
(1 College of Life Science, Shaanxi Normal University, Xi′an 710062, Shaanxi, China;2 Clinical Laboratory of Xi′an 630 Hospital, Xi′an 710000, Shaanxi, China)
Abstract:
The present study aims to investigate the cytotoxic effect of focused ultrasound combining HMME on human leukemia U937 cells. Tumor cells suspended in serum-free RPM1640 were exposed to ultrasound with 1 W/cm2 and 1.1 MHz for 60 seconds in the presence and absence of HMME. The viability of cells was determined by MTT. MTT results showed that the treatment with ultrasound (1.1 MHz, 2~7 W /cm2, 60 s) alone significantly inhibited the cell proliferation of the U937 cells in a intensity dependence,while the HMME (10~100 ng/L) alone showed no statistically significant differences with the control groups (P>0.05); Under the same conditions, the synergistic effect of 2 W /cm2 ultrasound combined with HMME showed significant cell killing compared with control (P
KeyWords:
ultrasound; Hematoporphyrin Monomethyl Ether（HMME）; U937 cells
