Abstract:
In order to study the oxidative damage of di(2-ethylhexyl)phthalate (DEHP) contaminated water to hepatic tissues of amphibious animal, male Rana chensinensises were exposed to DEHP acueous solution with concentrations of 10-7, 10-6, 10-5 and 10-4mol/L for treatment respectively for 20 d, 30 d and 40 d, then their livers were taken to prepare tissue homogenate, and the activity of superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT), glutathione -S- transferase (GST) and the content of malondialdehyde (MDA) and glutathione (GSH) were detected with spectrophotometic method. The results show that, compared with the control group, the activity of SOD and GPX declines, but that of CAT increases, and MDA content increases. The enzyme activities mentioned above gradually improve with the increase of DEHP concentration and extension of exposure time. However, the activity of GST decreases firstly and then increases with passing-by of exposure time, and GSH content shows an irregular change. The results demonstrate that the DEHP pollution in water may cause oxidative damage to frog′s hepatic tissue by means of impacting the activities of antioxidant enzymes, and the toxicity exhibits dose effect and cumulative effect.
