Polysaccharides (HNP) from Pleurotus tuber-regium were successfully modified by either sulfation, carboxymethylation, acetylation or phosphorylation, and their antioxidation and inhibition effects on α-amylase and α-glycosidase were studied. Results show that sulfation and carboxymethylation increased the antioxidant activity obviously, which the carboxymethylation modification effect was more significant. The scavenging hydroxyl radical and power were reduced but scavenging 1,1-diphenyl-2-picrylhydrazyl(DPPH·) radic and superoixide anion (O2-·) were improved after acetylation and phosphorylation. The modification products improved the inhibitory activity against α-amylase and α-glycosidase. Sulfation and carboxymethylation increased the anti-diabetic activity obviously and the carboxymethylation modification effect is better. The research shows that the antioxidation and inhibition effect on α-amylase and α-glycosidase of polysaccharides from Pleurotus tuber-regium can be improved remarkably through appropriate chemical modifications.

Pleurotus tuber-regium polysaccharide；chemical modification；antioxidation；α-amylase；α-glycosidase；inhibition ratio