Abstract:
The possible signal pathways involved in sonodynamic therapy (SDT) induced apoptosis in S180 tumor cells was explored. By using confocal microscope observation, western blotting and RT-PCR techniques, the cellular translocation and protein expression changes of some key apoptosis factors (AIF、Bid and NF-κB) were carefully investigated.The results showed that AIF translocates from plasma to nucleus in S180 cells post SDT treatment, however the protein and mRNA expression levels of AIF do not change. After ultrasound exposure, NF-κB is immediately activated and translocated from plasma to nucleus, simultaneously, and the protein and mRNA expression levels are significantly increased compared to the control. Whereas an obvious activated Bid is not observed. The results indicated that ultrasound and SDT may activate different cellular signal pathways to response to apoptotic stimulus. Ultrasound treatment can rapidly trigger cell apoptosis via NF-κB activation, whereas SDT treatment can induce mitochondria-dependent apoptosis in S180 cells.
