二氢卟吩e6光动力学疗法对食管癌Eca—109细胞增殖迁移的影响-陕西师范大学学报期刊社网站

陕西师范大学学报（自然科学版）

生命科学

二氢卟吩e6光动力学疗法对食管癌Eca—109细胞增殖迁移的影响

张坤1，崔连新1，康玲1,2，王筱冰1*，刘全宏1

（1 陕西师范大学生命科学学院，陕西西安710062； 2 新疆医科大学公共卫生学院，新疆乌鲁木齐 830011）

张坤，女，讲师，博士，主要研究方向为细胞生物学.E-mail：zkxian@stu.snnu.edu.cn.

摘要:

研究了二氢卟吩e6（Ce6）介导的光动力学疗法（PDT）对体外食管癌Eca—109细胞增殖迁移的影响.通过MTT法检测了Ce6-PDT对食管癌Eca—109细胞增殖的作用，采用划痕实验探究了Ce6-PDT对Eca—109迁移的影响.结果表明:无光照条件下，低浓度Ce6各组与对照组相比无显著性差异（P>0.05），但Ce6质量浓度为1.0 μg/mL时与对照组相比有显著性差异（P<0.05）；Ce6-PDT对Eca—109细胞增殖的抑制作用在一定范围内与Ce6浓度、光照剂量、作用时间和孵育时间成正相关（P<0.05）； Ce6-PDT对Eca—109细胞迁移能力的影响与未经处理的对照组比较作用不显著.可见Ce6-PDT能有效抑制Eca—109细胞的增殖，但Ce6-PDT对Eca—109迁移的作用不明显.

关键词：

二氢卟吩e6；光动力疗法；食管癌

收稿日期：

2013-01-10

中图分类号：

R730.57

文献标识码：

文章编号：

1672-4291(2013)05-0073-05

基金项目：

国家自然科学基金资助项目（81060186）；陕西师范大学勤助科研创新基金项目（QZZD11020）.

Doi:

Photodynamic effect of chlorin e6 on cell proliferation and migration of Esophageal Cancer Eca—109 Cells

ZHANG Kun1, CUI Lian-xin1, KANG Ling1,2, WANG Xiao-bing1*, LIU Quan-hong1

(1 College of Life Science, Shaanxi Normal University, Xi′an 710062, Shaanxi, China;2 College of Public Health, Xinjiang Medical University, Urumqi 830011, Xinjiang, China)

Abstract:

To explore the effects of Ce6-PDT on the proliferation and migration of esophageal cancer Eca—109 cells in vitro, MTT was applied to detect the effects of Ce6-PDT on the Eca—109 cell proliferation as well as cell migration combining with scratching techniques. The inhabitation effect was in a positive relation with Ce6 concentration, laser energy density, interaction time and incubation time within limits(P<0 .05), and statistically significant difference was found for the experimental groups and control groups in without laser only with high ce6 concentration (>1.0 μg/mL), and there is no evident effects of Ce6-PDT on Eca—109 cell migration for both the experimental groups and control groups; Ce6-PDT can effectively inhibit the Eca—109 cell proliferation but no evidently effects on migration.

KeyWords:

chlorin e6; photodynamic therapy; esophageal cancer