Abstract:
To explore the interference impacts of 4 glycolysis pathway genes on other meat quality related genes, liposome transfection technology was used to transfect siRNAs of 4 glycolysis pathway genes (PKM, PFKM, PGK1, ALDOA) into the isolated and cultured satellite cells of goat muscle. The mRNA expressions of 4 target genes and 15 meat quality related genes were detected by qPCR after induced differentiation, RNA extraction and reverse transcription. The results showed that there was no significant difference in cell proliferation characteristics and phenotypes between the control and the transfection groups, and the expression levels of 4 target genes decreased 33.5% to 93.8% after interference. The expressions of 7 meat quality related genes were significantly down-regulated and 5 genes were up-regulated after PKM interference, the expressions of 12 meat quality related genes were significantly down-regulated and 3 genes were up-regulated under the interference of PFKM and PGK1, and the expressions of 8 meat quality related genes were significantly down-regulated and 3 genes were up-regulated after ALDOA interference. The expression levels of LDHA, ACOX1, PNPLA, CAPN1 genes in the four transfection groups decreased significantly, while the expression levels of I-FABP, POMC, DGAT1 genes increased obviously. The above changed genes mainly involved in cellular energy metabolism, glucose and lipid metabolism, cell tissue structure formation pathways, among which lipid metabolism and energy regulation genes suffered most under the interference of the target genes. These results provide a reference for exploring the internal regulatory mechanism of meat quality formation at gene level.
