Abstract:
Rhamnogalacturonan-Ⅰ(RG-Ⅰ) is one of the key domains in plant pectin, and has received extensive attention due to its complex structures and diverse bioactivities. At present, the definition, separation and detection system of RG-Ⅰ are still unclear, which limits the activity research and functional product development of RG-Ⅰ, and restricts its industrial application. On this basis, the definition, structures, bioactivities, extraction, purification and detection of RG-Ⅰ are systematically summarized. RG-Ⅰ is proposed as a kind of pectic polysaccharide whose backbone is connected with rhamnose and galacturonic acid using α-1,4 glycosidic bond, and its rhamnose residues can be attached to neutral sugar side chains. Meanwhile, it is suggested that the sequential extraction in alkaline conditions may be a reasonable method for RG-Ⅰ extraction, and anion exchange chromatography combined with gel column chromatography could be a possible approach for RG-Ⅰ separation and purification. With RG-Ⅰ detection technology, monosaccharide composition is proposed as the main detection basis, and more comprehensive structures could be obtained combining with NMR, mass spectrometry and antibody verification.Through the summary, induction and prospect of RG-Ⅰ, it can provide reference for the follow-up study of RG-Ⅰ pectin polysaccharide.
