The middle and lower reaches of Heihe River Basin are important food-producing areas in Hexi Corridor, and the change of its food supply function has an important impact on the ecological construction and the healthy economic and social development of the basin. Based on the perspective of food supply, the ecosystem services such as water production, soil conservation, vegetation carbon sequestration and food supply in the middle and lower reaches of Heihe River Basin from 2000 to 2018 were calculated, the trade-off and synergy among them were discussed, the hot spots of food supply services were divided, and suggestions to improve the function of food supply were put forward. The results showed that the water production capacity was decreased from 2000 to 2018, but the soil conservation，carbon sequestration of vegetation and food supply capacity were increased. The relationship between ecosystem services is different in different land use types, but it is generally synergistic. The hot spot analysis showed that the fourth hot spots of food supply services occupy the largest area.The structure of hot spots has been optimized during the research, and the relationship between ecosystem services in different hot spots is obviously different. The research results play an important role in revealing the relationship between ecosystem services in the food supply area of Heihe River Basin and could improve the regional food supply capacity.

ecosystem services; trade-off and synergy; hot spot analysis; Heihe River Basin