Effect of phospholipid polymer coatings on blood compatibility of polypropylene membrane oxygenators
SHI Kehui1, WANG Yanbing2, LIU Wenyan1, ZHANG Huan1, GAO Julin1, GONG Yongkuan3*, JIANG Hongli1*
（1 Dialysis Department of Nephrology Hospital, First Affiliated Hospital of Xi′an Jiaotong University, Xi′an 710061, Shaanxi, China；2 Key Laboratory of Functional Textile Material and Product of Ministry of Education, School of Textile Science and Engineering, Xi′an Polytechnic University, Xi′an 710048, Shaanxi, China；3 Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi′an 710127, Shaanxi, China）
Abstract:
Three cell membrane mimetic phospholipid polymers, PMBT, PMLT and PMNC were coated on the surface of polypropylene (PP) membrane respectively, and the stability of polymer coatings and their effects on the blood compatibility of PP membrane oxygenator were studied. The dynamic contact angle measurement showed that no significant change was found in the dynamic contact angles of the three polymer coated PP membranes before and after being immersed in ethanol solution of different concentrations at different temperatures for a long time.In vitro protein adsorption experiments exhibited that the adsorption of bovine serum albumin and fibrinogen (FIB) on PMBT, PMLT and PMNC polymer coated PP membranes decreased significantly compared with that of the uncoated PP membrane. After the beginning of animal extracorporeal circulation (ECC), platelet count, leukocyte count and FIB concentration in the uncoated group decreased significantly,while the concentrations of β-thromboglobulin, interleukin-8, thrombin antithrombin complex, complement fragment 5a and prothrombin fragment F1+2 increased markedly. However, the above parameters in PMBT, PMLT and PMNC polymer coated groups did not change obviously during ECC and there was no significant difference among the three polymer coated groups. After 120 min of ECC, the oxygenator in the uncoated group displayed obvious coagulation, while the oxygenators in the three polymer coated groups showed no coagulation. These results suggest that PMBT, PMLT and PMNC polymer coatings not only possess excellent stability, but also significantly improve the blood compatibility of PP membrane oxygenator which provide an objective experimental basis for the further transformation and application of phospholipid polymer coating medical consumables.
KeyWords:
membrane materials; surface modification; blood compatibility; phospholipid polymer; polypropylene membrane; oxygenator
